1  Principle

Zeranol ELISA Kit is a competitive enzyme immunoassay for the quantitative analysis of Zeranol in corn, grain and feed, etc.
The test is a competitive direct ELISA that provides exact concentrations in parts per billion (ppb). Free toxin in the sample and controls competes with enzyme-labeled toxin (conjugate) for the antibody binding sites. After a wash step, substrate reacts with the bound enzyme conjugate to produce blue color. A stop solution is then add which changes the color from blue to yellow. The intensity of the color is inversely proportional to the concentration of Zeranol in the samples and standards.

2  Technical specification

Sensitivity:  0.50 ppb       

Detect time:  Approx.75 min

 
Detection limit:

Grains and Oil .......................... 1.0 ppb

Feed........................................... 2.0 ppb

Peanut ...................................... 1.5 ppb

Recovery rate:

85±10%

 
Cross-reaction rate:

Zeranol....................................... 100%

β-Zearalanol ............................... 60%

β-Zearalenol ............................... 70%

α-Zearalenol ............................... 85%