1  Principle

Histamine ELISA Kit is a competitive ELISA for the quantitative analysis of Histamine in the Fish, Shrimp tissue, etc. 

The coupling antigens are pre-coated on the micro-well stripes. The Histamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Histamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Histamine concentration in the sample. This value is compared to the standard curve and the content of the corresponding Histamine is subsequently obtained.

Enzyme immunoassay (ELISA) is widely used as a screening system, which is more simple, rapid, sensitive and cost-effective compared with HPLC or GC-MS.

2  Technical specification

Sensitivity:  2.5 ppm         

Detect time:  Approx.45 min

 
Detection limit:

Fish .............................................. 2.0 ppm 

Shrimp ........................................  2.5 ppm 

Recovery rate:

Fish .......................................... 85 ± 10%

Shrimp ..................................... 85 ± 15%

Cross-reaction rate:

Histamine ......................  100%