Instruction for the correct use of the RM
The compound should be stored at -20℃ in a dark place. Before usage of the RM , the compound should be allowed to warm to temperature(20±3℃).The recommended minimum sub-sample amount for all kinds of application is 1mg. Direct dissolution is recommended rather than a subsample dissolution.The expiry date of this RM is based on the current knowledge and holds only for proper storage conditions in the originally closed flasks/ packages.
Certified values and their uncertainties
AflatoxinM1 | ||
Compound | Purity | |
AflatoxinM1 | Certified value a | Uncertainty b |
98.50% | ±1.50% | |
a The certified value is based upon the results from several analytical techniques | ||
Purity assessment of AflatoxinM1
1 LC-MS/MS
The purity check using gradient LC-MS/MS of the AflatoxinM1 sample showed one main peak after blank subtraction.
2 HPLC-DAD/FLD
The purity check using LC-DAD of the Aflatoxin B1 sample showed one main peak after blank subtraction. The peak purity of the main signal was examined by diode array spectra of the AflatoxinM1 peak and led to the conclusion that this peak consists only of AflatoxinM1.
The purity check using LC-FLD of the AflatoxinM1 sample showed one main peak .