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A simple and efficient extraction and purification procedure for Fumonisins was developed by means of the immunoaffinity column (IAC-SEP®Fumo) as a cleanup tool．Fumonisins content in Rice, Corn, Wheat, Nuts, Spices, Peanuts Peanuts products, Vegetable oil, Feed samples are cleaned up by IAC and determined by HPLC or LC-MS/MS.