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A simple and efficient extraction and purification procedure for Aflatoxins was developed by means of the immunoaffinity column (IAC-SEP® AFLA) as a cleanup tool．Aflatoxins content in Grain, Food, Feeds, Nuts, Peanuts, Soy sauce, Vinegar, Chili, Pepper, Medicinal herbs and Wine samples are cleaned up by IAC and determined by HPLC or LC-MS/MS.