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A simple and efficient extraction and purification procedure for Aflatoxins was developed by means of the immunoaffinity column (IAC-SEP ® AFLA M1) as a cleanup tool．
This method do not use toxic solvents such as chloroform and methylene chloride. AflatoxinM1 content in milk, fermented milk, powder, infant formula powder, cheese and cream samples are cleaned up by IAC and determined by HPLC or LC-MS/MS.