1. Principle

Sudan red ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Sudan red in egg, feed and food.

The coupling antigens are coated on the micro-well stripes. The Sudan red in the  sample and the coupling antigens coated on the micro-well stripes compete for the anti-Sudan red antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.

The optical density (OD) value of the sample has a negative correlation with the Sudan red in it. The measurement is made photometrically at 450nm. The absorption is inversely proportional to the Sudan red concentration in the sample.

2. Technical specifications

Sensitivity : 0.1 ppb     

Detect time: Approx. 75 min

Detection limit:

  Eggs ..............................................................10 ppb

  Tomato Juice / Chili sauce / Ketchup ............20 ppb  

  Feed / Paprika...............................................100 ppb    

Recovery rate:

  Eggs ..........................................................70 ± 10%    

  Tomato Juice/Chili sauce/Ketchup.............80 ± 10%

  Feed / Paprika............................................65 ± 10%

Cross-reaction rate:

  Sudan red.................................................... 100%

  Para Red.......................................................123%

 Rhodamine B....................................................8%