Principle

Oxolinic Acid ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Oxolinic Acid in fish, shrimp and tissue.

The coupling antigens are coated on the micro-well stripes. The Oxolinic Acid in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Oxolinic Acid antibodies. After the addition of the conjugated antibody, the TMB substrate is added for coloration.

The optical density (OD) value of the sample has a negative correlation with the Oxolinic Acid in it. The measurement is made photometrically at 450nm. The absorption is inversely proportional to the Oxolinic Acid concentration in the sample.

Technical specification:

Sensitivity: 1.0 ppb      Detect time: Approx.45 min

Detect limit:

Tissue: 1 ppb ，Serum and honey :2ppb

Recovery rate:

Tissue........................................... 85 ± 15 %    

Serum and honey......................... 90 ± 10 %  

Cross-reaction rate:

Oxolinic Acid.......................................100％