1. Intended Use
A simple and efficient extraction and purification procedure for Folic acid was developed by means of the immunoaffinity column (IAC - SEP ® FA) as a cleanup tool. This method do not use toxic solvents such as chloroform and methylene chloride. Folic acid content in drink, milk, milk powder are cleaned up by IAC and determined by HPLC or LC-MS/MS. It is a fast,simple, safe and highly accurate method for quantitatively measuring Folic acid.
2. Principle
Samples are prepared by mixing with an extraction solution(or just centrifuging), blending and filtering.The extract is then applied to the Folic acid immunoaffinity column bound with specific antibodies to Folic acid．At this stage, the Folic acid bind to the antibody on the column.The column is then washed with water to remove the impurities. By passing 30 % Acetonitrile Containing 0.2 % TFA through the column, the Folic acid is removed from the antibody. This methanol solution can then be injected into HPLC or LC-MS/MS system.