Assay Pinciple

This instruction of simultaneous Aflatoxins determination in food and feed focuses on the enrichment step of extract using immunoaffinity column (IAC) and quantification with HPLC. Accepted laboratory extraction methods could be maintained. Full performance of the IAC column is given if pronounced criteria regarding organic solvent tolerance, elution process of analyte and working range of column is followed. Many pretreatment methods of Aflatoxin determination in food and feed show low sensitivity because of interfering substances if problematic matrices are applied. This method of content determination of Aflatoxins combines the high selectivity of an immunoaffinity column (IAC) with its potential to concentrate eluate and additional step of purification by HPLC column.

Please notice that this instruction focuses on the handling with the IAC column. For the commodity extraction step a literature method is given. The given apparatus (e.g. HPLC system) might serve as example among other possibilities.

Cross Reactivity

Aflatoxin B1----100%       

Aflatoxin B2----80%

Aflatoxin M1----30%

Aflatoxin G1----100%  

Aflatoxin G2----140%          

Aflatoxin M2----35%  

Technical Specification                                                                                  

Reaction temperature: 25℃    

Detect time:  60 min.

Package: 3mL, 25 columns