1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Phenylethanolaminea in the pork, liver, urine. The coupling antigen is pre-coated on the micro-well stripes. The Phenylethanolaminea in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Phenylethanolaminea antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Phenylethanolaminea in it. This value is compared to the standard curve and the Phenylethanolaminea residues is subsequently obtained.

2. Technical specifications

Sensitivity:  0.10ppb

Detection limit

Tissue...................................0.5ppb

Urine, serum........................0.5 ppb

Feed......................................1 ppb

Recovery rate

Urine, serum.......................90±10%

Tissue.................................80±10%

Feed....................................80±15%

Cross-reaction rate

Phenylethanolaminea...........100%

Terbutalin...........................< 0.1%

Clenbuterol........................< 0.1%

Dobutamine......................< 0.1%