1. Principle

Malachite green ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Malachite green in tissue, feed and water.The coupling antigens are coated on the micro-well stripes. The Malachite green in the  sample and the coupling antigens coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.

The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Malachite green concentration in the sample.

2. Technical specifications

Sensitivity : 0.05 ppb          Detect time: Approx. 75 min

Detection limit:

Tissue and water.........................................0.5 ppb

Feed................................................................1.0 ppb

Recovery rate:

Tissue and feed..........................................75 ± 10%

Water........................................................... 80 ± 20%

Cross-reaction rate:

Malachite green..............................................100%

Leucomalachite Green ...................................100%

Crystal Violet ................................................. 95%

Leucocrystal Violet .........................................95%