Test principle

The basis of the test is on the competitive enzyme immunoassay for the detection of Aristolochic Acids in the serum and perilymph sample. The microtiter wells are coated with anti-Aristolochic Acids antibody. Aristolochic Acids enzyme conjugate and the Aristolochic Acids standards or sample solution are added. Free Aristolochic Acids antibody and Aristolochic Acids enzyme conjugate compete for the Aristolochic Acids antibody binding sites (competitive enzyme immunoassay). Any unbound enzyme conjugate is then removed in a washing step. Substrate solutions are added to the wells, bound enzyme conjugate converts the colorless chromogen into a blue product. The addition of the stop reagent leads to a color change from blue to yellow.The measurement is made photometrically at 450nm. According to the absorbance, the concentration of Aristolochic Acids in the sample can be quantified.

Technical specification:

Sensitivity: 2.0ppb

Detect time: Approx.45 min

Detect limit:

Tissue..........................................10ppb

Serum,Urine ...............................5ppb

Recovery rate:

Tissue..........................................85 ± 15%

Serum,Urine.................................85 ± 20%

Cross-reaction rate:

Aristolochic Acids............................100%