Summary

Prestained Protein Ladder is a prestained mixture of ten recombinant proteins ranging from 10 kDa to 180 kDa. Three different chromophores are bound to the proteins, producing a brightly colored ladder. The protein ladder is conveniently packaged and ready to use with no heating, diluting or additional reducing agent necessary. Lot-to-lot variation of the apparent molecular weight of prestained proteins is ~5 %.

Storage

Store at -20 ℃.

Specification

Recommendations for Loading

1.  Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil!

2.  Mix gently, but thoroughly, to ensure the solution is homogeneous.

3.  Load the following volumes of the ladder on an SDS-polyacrylamide gel:

    – 5 μL per well for mini gel,

    – 10 μL per well for large gel.

    Use the same volumes for Western blotting.

   The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. The loading        volume should be doubled for 1.5 mm thick gels.

PageRuler Prestained Protein Ladde

Note:

The apparement molecular weight of each protein (KDa) has been
determined by calibration against an unstained protein ladder in each electrophoresis condition.

Supplement data should be considered for more accurate adjustment.

Migration Pattems of PageRuler Prestained Protein Ladder
                                            □ 1NGM001  □ 15%SDS-PAGE    □ 4%~20%