Principle

SuperLumia ECL Plus HRP Substrate Kit is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed. The special formulation of ECALBIO SuperLumia ECL Plus HRP Substrate makes it the ideal substitute for Pierce ECL Western Blotting Substrate without the need for additional optimization of assay conditions.

                   

                                   The principle of chemiluminescent Western Blotting

 





             

                                               Chemiluminescence of luminal

Kit Contents

SuperLumia ECL Plus HRP Substrate Kit Contains sufficient substrate for 1200 or 2500 cm² of membrane surface. The following components are included: 

Storage

Store at 2-8 ℃.