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Western Blot Assay Kit is also called Protein Carbonyl Assay Kit, designed for the measurement of protein carbonyl groups that are created by the oxidation of proteins. This can be through reaction with ozone or oxides of nitrogen, or by metal catalyzed oxidation.
In the protein carbonyl assay protocol, carbonyl groups in protein side chains are derivatized to DNP-hydrazone by reaction with DNPH. The DNP moieties are detected using an anti-DNP antibody and western blotting.