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Annexin V-FITC/PI Apoptosis Assay Kit is using FITC-label- Annexin V protein to detect cell apoptosis.
Annexin V is a calcium-dependent phosphatidylserine binding protein with a high affinity for phosphatidylserine PS. Annexin V labelled FITC can bind to the membrane of early apoptotic cells by means of the PS exposed outside the cells. Apoptosis can be detected by flow cytometry or fluorescence microscopy.