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Situ TUNEL Apoptosis Assay Kit applies a highly sensitive, fast and simple method to detect cell apoptosis. The results can be directly observed through a fluorescence microscope.
The cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP.