1.  Principle

Florfenicol ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Florfenicol in milk, honey, tissue and serum.

The coupling antigens are coated on the micro-well stripes. The Florfenicol in the sampleand the coupling antigens coated on the micro-well stripes compete for the anti-Florfenicol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Florfenicol in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Florfenicol concentration in the sample.

2.  Technical specification:

Sensitivity: 0.10ppb      Detect time: Approx.45 min

Detect  limit:

Chicken, duck, pork, shrimp, fish ........ 0.30ppb

Honey................................................ 0.30 ppb

Feed...................................................1.20 ppb

Serum and milk ..................................2.0 ppb

Cross-reaction rate:

Florfenicol............................................100%

Florfenicol Amine ..................................15%

Chloramphenicol................................... <1%

Thiamphenicol...................................... <1%

Recovery rate:

Tissue ....................................... 85 ± 10%

Honey, milk ................................ 85 ± 15%

Feed .......................................... 75 ± 10%