1. Principle
Colistin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Colistin in the chicken, duck, pork, shrimp, fish, honey and milk sample.
The coupling antigens are coated on the micro-well stripes. The Colistin in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Colistin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Colistin in it. The measurement is made photometrically at 450nm. The absorption is inversely proportional to the Colistin concentration in the sample.
2. Technical specification:
Sensitivity: 1.0ppb Detect time: Approx. 45min
Detect limit:
Chicken, duck, pork, shrimp, fish .............. 5.0ppb
Honey and milk..........................................20ppb
Cross-reaction rate:
Colistin.................................................. 100%
Recovery rate:
Tissue ..................................... 85±20%
Honey and milk......................... 80±20%