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Human IgG1 kappa Isotype Control

Type: Human Monoclonal Antibody

Isotype: Human IgG1, kappa light chain

Specification:0.5mg, 25mg

Clone ID:    E34
Molecular Weight:  145.97 kDa

Description

Type:Human Monoclonal Antibody

Isotype: Human IgG1, kappa light chain

Clone ID:E34

Molecular weight: 145.97kDa


Package Size

Cat NO.

Specification

Volume

Concentration

91001-05

0.5 mg

100μL

5μg/μL

91001-25

25 mg

25*200μL

5μg/μL


Applications:

This IgG1/kappa isotype antibody can be used as the negative antibody control in any cell based or animal based assay. For example, this antibody can be used as negative control in cell based T cells activation or ADCC assay(Please refer to Fig1 and 2 in the attachment).


Please Note: Optimal concentrations/dilutions should be determined by the end user.

Preparation: CHO production

Purification: Protein A, CEX


Formulation:

0.2μm filtered solution in 100mmol NaCl, 10mmol Tri-sodium citrate dihydrate, 0.02% Tween 80,PH6.0.


Storage:

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃ . Preservative-Free.


QC Testing

Character

Method

Result

Antibody concentration

UV

10mg/mL

Aggregation

SEC-HPLC

< 2%

Purity

SEC-HPLC

>98%

Endotoxin

LAL gel clotting

< 3 EU/mg



 Figures1: T cells activation asssy with IgG1 as negative control


 Figures2: ADCC assay with IgG1 as negative


                            Figures3 :Purity of IgG1 by SEC-HPLC

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