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Nucleic Acid Release Reagent

Product Principle

Nucleic Acid Release Reagent provide a simple, safe and effective method to release nucleic acid from clinical samples containing pathogens.


Model and Package Specification
Model specification: 3 mL/tube. 
Package specification: 1 tube-100 tubes/box. 

Description

Product Principle
Nucleic Acid Release Reagent provide a simple, safe and effective method to release nucleic acid from clinical samples containing pathogens. By dissolving protein, the secondary structure of protein disappears, leading to the structural degradation of cell or virus, and then the nucleic acid rapidly separates from the nucleoprotein. This reagent can quickly release nucleic acid from freshly collected clinical samples, and protect nucleic acid from degradation.
Clinical samples preprocessed by Nucleic Acid Release Reagent can be directly used for molecular detection, such as PCR, RT-PCR, etc., without heating or other nucleic acid extraction operations of samples.

Intended Use
Nucleic Acid Release Reagent is used for the pretreatment of the samples, so that the nucleic acid to be tested can be released from the binding state with other substances. In this way, nucleic acid can be easily and directly detected. The crude lysates can be directly applied in polymerase chain reaction (PCR) or RT-PCR.

Model and Package Specification
Model specification: 3 mL/tube.
Package specification: 1 tube-100 tubes/box.

Storage Conditions and Validity
The product can be stored at 2-35°C for 12 months before use, and can be transported at 2-35°C for a long time.

After use, the sample can be preserved in the product at room temperature for 1 week. If longer preservation time is needed, please store the sample below -15℃.


Application

1.  Swab samples

(1) Clean hands before sampling, tear off the outer packaging of the swab, and take the swab out. Be careful not to touch the swab head. Use a swab to sample the corresponding part according to different sampling requirements.

(2) After sampling, quickly put the swab into the sampling tube containing nucleic acid release reagent to avoid contact with other parts.

(3) Break the swab along the grooves in the swab rod, discard the tail, tighten the cap, and complete the sampling.

(4) After sampling, turn the tube upside down manually and forcefully for 1 second, and then let stand for 5 minutes, the nucleic acid is released into the release agent.

 

2.  Sputum, saliva, drawing sample

(1) Sample according to different sampling requirements.

(2) Place the collected sample into the sample tube containing the nucleic acid release agent to complete the sampling.

Note:The volume of sample shouldn’t exceed 20% of the volume of the Nucleic Acid Release Agent.

(3) After sampling, turn the tube upside down manually and forcefully for 5 seconds, and then let stand for 5 minutes, the nucleic acid is released into the release agent.


3.  The volume of the sample treated with Nucleic Acid Release Reagent should not exceed 30% of the total reaction volume of PCR . And PCR should be conducted within half an hour after adding the treated sample.

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