This test kit is a competitive ELISA for the quantitative analysis of Rapamycin in the tissue, urine, blood,etc. The coupling antigens are pre-coated on the micro-well stripes. The Rapamycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Gen antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Rapamycin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Rapamycin is subsequently obtained.
Sensitivity : 5ppb
Detect time: Approx. 45 min
Tissue, liver, intestine,milk .................... 5.0ppb
Serum，medium solution ...................... 100ppb
Recovery rate:
Tissue, liver, intestine,milk ....... 85±10%
Cross-reaction rate:
Rapamycin..................... 100 %
Tacrolimus......................<1%