1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the tissue, liver, honey, intestine, shrimp, fish, milk and feed etc. The coupling antigens are pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-CAP antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Chloramphenicol concentration in the sample. This value is compared to the standard curve and the content of the corresponding Chloramphenicol is subsequently obtained.
2. Technical specifications
Sensitivity : 0.025 ppb
Detect time: Approx. 45 min
Detection limit:
Urine, honey, intestine .......................0.05 ppb
Tissue, liver, milk .......................... ....0.025 ppb
Feed .......................... .......................0.075 ppb
Recovery rate:
Tissue, liver ....................................... 80±10%
Honey, urine, intestine,serum .............70±10%
Milk,feed ............................................ 85±15%
Cross-reaction rate:
Chloramphenicol.....................100%
Thiamphenicol.........................<0.1%
Florfenicol...............................<0.1%