The unique binding solution/protease K rapidly lysates cells and inactivates intracellular nucleases, and the DNA is selectively adsorbed to the silicon matrix membrane of spin column in a state of high dissequencing salt, removing cell metabolites, proteins and other impurities through rapid rinse-centrifugation steps, inhibitor removal solution and bleaching solution. Finally, a low-salt washing buffer elutes the pure DNA from silicon substrate membrane.
Features
- Don’t use phenol and toxic reagents, don’t need ethanol precipitation in whole process.
- Fast and simple, a single sample is extracted within 20 minutes.
- Multiple column rintions ensure high purity. 3-12µg of whole blood can be extracted with a typical output of 200µL. The typical ratio of OD260/OD280 can reach 1.7-1.9, and the length can reach 30kb-50kb, which can be directly used for PCR, Southern blot and various enzyme digestion reactions.
- The red blood cell lysate formula is selected from more than a dozen formulas, which can be split quickly and completely.
- 3-6µg DNA can be extracted from the typical 200µL whole blood.
Intended use
Genomic DNA Purification Kits are intended for molecular biology applications. The kits are not used in the diagnosis, prevention, or treatment of a disease.