Description
Recombinant bovine enterokinase is a highly purified bovine enterokinase light chain. Like natural bovine enterokinase, recombinant bovine enterokinase can specifically recognize the DDDDK amino acid sequence in the protein sequence and cut at the C-terminal. Recombinant bovine enterokinase has higher activity compared with natural bovine enterokinase.
The characteristics Recombinant enterokinase is high activity, high specificity, and no excess amino acid in the N terminal after cutting the target protein, and is often used in the label removal of recombinant fusion protein. This product has better activity in the condition of 4-37℃ with pH5-9.
Format: Purified, Lyophilized
Features
|
Product Name |
Recombinant Bovine Enterokinase(Lyophilized), rbEK(Lyo) |
|
Origin |
E. coli |
|
Molecular Weight |
29.3kDa |
|
Isoelectric Point |
5.48 |
|
Purity |
≥95%（SDS-PAGE） |
|
A280nm(1mg/mL) |
2.212 |
|
Specific Activity |
≥50kU/mg |
|
Formation |
Lyophilize，product is 20mM Tris-HCl(pH8.0) before being lyophilized |
|
Activity Definition |
Buffer（20mM Tris-HCl, 0.1% Tween-20, 50mM NaCl, 1mM CaCl2, pH8.0）Bin enzyme digestion，react for 16h at 25，volume of enzyme required to shear >95% of 50μg substrate is defined as one unit of activity (U). |
|
Storage and Expiration date |
-20±5℃ for 3 years |
|
Shipping Process |
Room or cool temperature(4-25℃） |