This test kit is based on the competitive enzyme immunoassay for the detection of Sirolimus in the tissue, honey, intestine, shrimp, fish, etc. The coupling antigens are pre-coated on the micro-well stripes. The Sirolimus in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Gen antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Sirolimus concentration in the sample. This value is compared to the standard curve and the content of the corresponding Sirolimus is subsequently obtained.
2. Technical specifications
Sensitivity : 1 ppb
Detect time: Approx. 75 min
Tissue, liver, intestine,milk ....................10 ppb
Recovery rate:
Tissue, liver, intestine,milk ....... 85 ±10%
Cross-reaction rate:
Sirolimus.......................100 %
Tacrolimus......................<1%