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Tilmicosin ELISA Test Kit

Tilmicosin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Tilmicosin in milk, honey, tissue ans serum.
The OD value is compared to the standard curve and the content of the corresponding Tilmicosin is subsequently obtained.

Sensitivity: 0.50ppb
Specification: 96tests

Description

1.  Principle
Tilmicosin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Tilmicosin in milk, honey, tissue and serum.
The coupling antigens are coated on the micro-well stripes. The Tilmicosin in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Tilmicosin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tilmicosin in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Tilmicosin concentration in the sample.

2.  Technical specification:
Sensitivity: 0.50ppb      Detect time: Approx.45 min

Detect  limit:
Chicken, duck, pork, shrimp, fish .............. 3.0ppb
Honey....................................................... 1.50 ppb
Serum and milk ........................................ 10 ppb

Cross-reaction rate:
Tilmicosin...............................................100%
Erythromycin .........................................1%

Recovery rate:
Tissue ........................................ 85 ± 10%
Honey, milk ................................ 85 ± 15%

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