Testosterone ELISA Kit

Testosterone ELISA Kit is a competitive enzyme immunoassay for the quantitative analysis of Testosterone in the samples such as tissue, serum or other samples.

The OD value is compared to the standard curve and the content of the corresponding Testosterone is subsequently obtained.

Sensitivity: 0.1ppb

Specification: 96tests/kit

Description

Principle

The method is based on a competitive colorimetric ELISA assay. The Testosterone-BSA has been coated in the plate wells. During the analysis, standards or sample is added along with the HRP-Conjugated Antibody and Testosterone Antibody. If the Testosterone residue is present in the sample, it will compete for the Testosterone antibody, thereby preventing the Testosterone-BSA from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Testosterone residue concentration in the sample.

Linear Interval: 0. 1ng/mL~8. 1ng/mL

Detection Time: 45min

Limit of Quantition, LOQ

Meat(Fish,Shrimp,Chicken,Beef,Pork)	0.8
Milk	0.3
Urine	0.2
Plasma/Serum	0.25

Sample Type	LOQ(ng/mL or ppb)
Meat(Fish,Shrimp,Chicken,Beef,Pork)	1
Milk	0.5
Urine	0.3
Plasma/Serum	0.5

Specificity ( Corss-Reactivity)

Analytes	Cross-Reactivity(%)
Testosterone	100
Boldenone	67
methyltestosterone	Less than 1
Cortisol	Less than 1
Progesterone	Less than 1

Accuracy( Spiked Recovery)

Sample Type	Spiked Recovery(%)
Meat(Fish,Shrimp,Chicken,Beef,Pork)	100±20
Milk/plasma/Serum	100±15
Urine	100±20

Storage and Shelf Life

Store the kit at 2-8℃; The shelf life is 12 months when the kit is properly stored.

Sample Preparation

Extraction Diluent

Mix 50mL of methanol with 950mL distilled water, ready for use.

Meat（Fish,Shrimp,Beef,Chicken,Pork）

1. Homogenize a reasonable amount of sample with a suitable mixer.

2. Weigh out 1g of the homogenize sample into a suitable vial and mix thoroughly with 4mL of Extraction Diluent, vigorously vortex for 2min.

3. Centrifuge it at 4,000 x g for 5 minutes.

4. Use 50μL of the supernatant per well for the assay.

Note: Dilution Factor :4

Milk

1. High-fat milk should be defatted by centrifuging;

2. Pipette 2mL of the milk sample and add 0.5mL of methanol, vortex for 15s

3. Use 50μL per well for the assay.

Note:Dilution Factor:1.25

Urine

1. Centrifuge 1 mL of the urine sample at 4,000 x g for 5 minutes.

2. Pipette 0.5mL of the urine supernatant and mix with 0.4mL of distilled water and 0. 1mL of methanol, vortex for 15s;

3. Use 50μL per well for the assay.

Note: Dilution Factor:2

Plasma/Serum

1. Pipette 2mL of the homogenize sample into a suitable vial and mix with 0.5mL of methanol ,vortex for 15s.

2. Use 50μL per well for the assay.

Note: Dilution Factor: 1.25

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