1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Capsaicin in the rapeseed oil, soybean oil, peanut oil, corn oil, blended oil, etc. The coupling antigens are pre-coated on the micro-well stripes. The Capsaicin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Gen antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Capsaicin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Capsaicin is subsequently obtained.

2. Technical specifications

Sensitivity : 0.10µg/kg

Detect time: Approx. 75 min

Detection limit:

Rapeseed oil, soybean oil, peanut oil, corn oil, blended oil ................ 0.40µg/kg

Recovery rate:

Rapeseed oil, soybean oil, peanut oil, corn oil, blended oil ................. 85 ±10%

Cross-reaction rate:

Capsaicin...............................100 %

Dihydrocapsaicin...................100 %

Nonivamide............................100 %