1.  Principle

This test kit is based on the competitive enzyme immunoassay for the detection of  Vancomycin in the tissue, liver, honey, intestine, shrimp, fish, etc. The coupling antigens are pre-coated on the micro-well stripes. The Vancomycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Gen antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with  Vancomycin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Vancomycin is subsequently obtained.

2.  Technical specifications

Sensitivity : 1 ppb

Detect time: Approx. 75 min

Detection limit:

Tissue, liver, intestine,milk .........................20 ppb

Recovery rate:

Tissue, liver, intestine,milk ....... 85 ±10%

Cross-reaction rate:

Vancomycin.......................100 %