PMA as a new DNA modification agent, can selectively combine with DNA in dead cells to form a complex, while DNA in living cells is not modified by PMA and can be amplified by qPCR . The PMA-qPCR Kit can selectively distinguish DNA in active cells from dead cells, and then perform qPCR reactions. It is widely used in food testing, medical testing and environmental testing, to improve the accuracy of detection.
Principle of PMA-qPCR Kit
PMA embedded double-stranded DNA in dead cells formed modified PMA-DNA under the action of light, which could not be amplified by PCR. Since PMA-DNA cannot cross cell membranes, DNA in living cells is not modified by PMA, and live bacteria can be selectively detected by quantitative PCR.
Live and heat-inactivated E. coli were detected by PMA-qPCR Kit
(A) Real-time PCR quantitative analysis of live and dead Escherichia coli pretreated with PMA, using paragraph of 16SrRNA.
(B) CT of live and dead bacteria samples treated with PMA and those not treated with PMA. Ct is calculated by subtracting the CT value of the sample treated with PMA from the corresponding sample treated without PMA (i.e. Ct with PMA - Ct without PMA).
Escherichia coli PMA-qPCR Kit
Cronobacter PMA-qPCR Kit
Escherichia coli PMA-qPCR Kit
STEC PMA-qPCR Kit
Lactobacillus PMA-qPCR Kit
Bifidobacterium PMA-qPCR Kit
Streptococcus thermophilus PMA-qPCR Kit
Vibrio parahaemolyticus PMA-qPCR Kit
Yersiniavan Loghem PMA-qPCR Kit
Campylobacter jejuni PMA-qPCR Kit
Salmonella PMA-qPCR Kit
Streptococcus PMA-qPCR Kit
Clostridium Botulinum PMA-qPCR Kit
Clostridium perfringens PMA-qPCR Kit
Bacillus cereus PMA-qPCR Kit
Bongkrekic Acid PMA-qPCR Kit
Listeria monocytogenes PMA-qPCR Kit
Lactobacillus rhamnosus PMA-qPCR Kit
Clostridium difficile PMA-qPCR Kit